Microcrystallization method use for assessing the content of secondary metabolites of lichens

The paper presents photographs of crystals fumarprotocetraric, evernic, usninic, solorinic, olivetoric, perlatolinic, physodalic, salazinic, tamnolic acids and atranorin obtained from their acetone extracts lichens. shape the is specific to each substance, number, size degree aggregation depend on concentration in solution. Stellate have atranorine, salazinic evernic acids, with atranorine having largest salacic acid smallest; rodshaped branched form solorinic fumarprotocetraric wider first, but longer second; oval larger small narrow perlatolinic acid; usnic, physodalic olivetoric unique crystal forms prismatic, needle pinnate, respectively. It possible observe secondary metabolites lichens a conventional light microscope under 40 lens without immersion oil. For subsequent comparative assessment amount substances, it necessary standardize suspension studied lichen thallus, use strictly fixed volume acetone, view at least four sides dried drop slide identify total number points, noting presence, branching into conglomerates.